rnase v1 Search Results


rnase v1  (Promega)
90
Promega rnase v1
Rnase V1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
rnase v1 - by Bioz Stars, 2026-05
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rnase v1  (Amersham Pharmacia Biotech Ltd)
86
Amersham Pharmacia Biotech Ltd rnase v1
The PV 5′ UTR. (A) Schematic representation of the predicted secondary structure of the PV 5′ UTR. The shaded box represents the oligopyrimidine tract. (B) Detailed view of domain V and the oligopyrimidine tract for the Leon strain of PV. The mutation involved in attenuation of Sabin3 is boxed. Nucleotides susceptible to <t>RNase</t> ONE cleavage in the Sabin3, but not the Leon, IRES are indicated by triangles (see Fig. ​Fig.2A).2A). Nucleotides protected by PTB or nPTB from <t>V1</t> cleavage are indicated by circles (see Fig. ​Fig.2B),2B), and nucleotides protected by PTB or nPTB from RNase ONE cleavage are indicated by squares (see Fig. ​Fig.33).
Rnase V1, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnase v1/product/Amersham Pharmacia Biotech Ltd
Average 86 stars, based on 1 article reviews
rnase v1 - by Bioz Stars, 2026-05
86/100 stars
  Buy from Supplier

Image Search Results


The PV 5′ UTR. (A) Schematic representation of the predicted secondary structure of the PV 5′ UTR. The shaded box represents the oligopyrimidine tract. (B) Detailed view of domain V and the oligopyrimidine tract for the Leon strain of PV. The mutation involved in attenuation of Sabin3 is boxed. Nucleotides susceptible to RNase ONE cleavage in the Sabin3, but not the Leon, IRES are indicated by triangles (see Fig. ​Fig.2A).2A). Nucleotides protected by PTB or nPTB from V1 cleavage are indicated by circles (see Fig. ​Fig.2B),2B), and nucleotides protected by PTB or nPTB from RNase ONE cleavage are indicated by squares (see Fig. ​Fig.33).

Journal:

Article Title: Molecular Mechanisms of Attenuation of the Sabin Strain of Poliovirus Type 3

doi: 10.1128/JVI.78.20.11097-11107.2004

Figure Lengend Snippet: The PV 5′ UTR. (A) Schematic representation of the predicted secondary structure of the PV 5′ UTR. The shaded box represents the oligopyrimidine tract. (B) Detailed view of domain V and the oligopyrimidine tract for the Leon strain of PV. The mutation involved in attenuation of Sabin3 is boxed. Nucleotides susceptible to RNase ONE cleavage in the Sabin3, but not the Leon, IRES are indicated by triangles (see Fig. ​Fig.2A).2A). Nucleotides protected by PTB or nPTB from V1 cleavage are indicated by circles (see Fig. ​Fig.2B),2B), and nucleotides protected by PTB or nPTB from RNase ONE cleavage are indicated by squares (see Fig. ​Fig.33).

Article Snippet: RNA transcripts were incubated for 10 min at 37°C with greater-than-equal molar amounts of PTB, nPTB, and Nova-1 to form complexes and then probed with RNase ONE (Promega) or RNase V1 (Amersham Pharmacia Biotech) essentially as previously described ( 23 , 42 ).

Techniques: Mutagenesis

PTB and nPTB bind the Leon and Sabin3 IRESs at a site adjacent to nt 472. Enzymatic footprint of the Leon and Sabin3 IRESs in the region surrounding nt 472 with RNase ONE (A) and RNase V1 (B). In this figure and the subsequent two figures, the table above the gel indicates whether RNA was incubated alone or with proteins and whether RNA-protein complexes were digested with an enzyme. RNA probes were extended with a primer complementary to nt 542 to 559. The region displaying enhanced cleavage by RNase ONE on the Sabin3 strain is indicated by the dashed box (see also triangles in Fig. ​Fig.1B).1B). The location of the PTB- or nPTB-binding site is indicated by the solid box (see also circles in Fig. ​Fig.1B1B).

Journal:

Article Title: Molecular Mechanisms of Attenuation of the Sabin Strain of Poliovirus Type 3

doi: 10.1128/JVI.78.20.11097-11107.2004

Figure Lengend Snippet: PTB and nPTB bind the Leon and Sabin3 IRESs at a site adjacent to nt 472. Enzymatic footprint of the Leon and Sabin3 IRESs in the region surrounding nt 472 with RNase ONE (A) and RNase V1 (B). In this figure and the subsequent two figures, the table above the gel indicates whether RNA was incubated alone or with proteins and whether RNA-protein complexes were digested with an enzyme. RNA probes were extended with a primer complementary to nt 542 to 559. The region displaying enhanced cleavage by RNase ONE on the Sabin3 strain is indicated by the dashed box (see also triangles in Fig. ​Fig.1B).1B). The location of the PTB- or nPTB-binding site is indicated by the solid box (see also circles in Fig. ​Fig.1B1B).

Article Snippet: RNA transcripts were incubated for 10 min at 37°C with greater-than-equal molar amounts of PTB, nPTB, and Nova-1 to form complexes and then probed with RNase ONE (Promega) or RNase V1 (Amersham Pharmacia Biotech) essentially as previously described ( 23 , 42 ).

Techniques: Incubation, Binding Assay